Collect the cells located between the two density layers . the intermediate zone was enriched of mscs 建议在取单个核细胞层时,亦收集此细胞层上下附近的液体,因为其中也含有单个核细胞。
In many lakes in summer that is a warm low - density layer ( epilimnion ) lying above a colder denser layer ( the hypolimnion ) , the zone of rapid temperature change between the two layers being celled the thermocline 夏季,多数湖中温暖的低密度水层(湖面温水层)在寒冷的较高密度水层(湖底净水层)之上,中间夹有温度改变较快的水层(变温层) 。